Deoxyribonuclease I

Category:Excipients > Other Excipients
Product Name:Deoxyribonuclease I
CAS No.:/
Standard:In-house Standards
Price(USD):0.00
Company:Ningbo Linzyme Biosciences Co., Ltd.

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Basic Info

Grade: Pharmaceutical Grade

Factory Location: Shanghai

Main Sales Markets: Western Europe,Eastern Europe,Asia
Sample Provided: no

Shanghai Linzyme Biosciences Ltd.Deoxyribonuclease I - Shanghai Linzyme Biosciences Ltd.

Source: Bovine Pancrease
EC: 3.1.21.1

DESCRIPTION
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8. DNase I is activated by bivalent metals, and inhibited by chelating agents such EDTA and sodium dodecyl sulfate. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.
Linzyme Deoxyribonuclease has been chromatographically purified to remove trace contaminating proteases. DNase may be used in clinic to thin and liquefy mucus in the lungs.

SPECIFICATION (I)

Item	Specifications	Methods
Appearance	White or pale yellow lyophilized powder
Activity	≥ 3,000 Christensen units/mg powder	K.P.
Loss on drying	≤ 0.3%	K.P.
Residue on ignition	≤ 1.0%	K.P.

Conclusion: Product complies with KP.

SPECIFICATION (II)

Activity

≥ 500 Kunitz units/mg powder

Sigma-aldrich

SPECIFICATION (III)

Activity

≥ 2,000 Kunitz units/mg powder

Sigma-aldrich

STORAGE
Store in an airtight container, at a temperature of 2°C to 8°C.

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